Nucleic Acid Extraction Scheme
At present, a large number of Reliable Nucleic Acid Extraction Reagent for novel coronavirus have been used in the frontline of the epidemic, but the efficiency of prevention and control and detection has not been significantly improved. CCTV reporter interviewed frontline laboratory staff in the report, also pointed out that there are differences in nucleic acid extraction links, affecting the improvement of detection ability. In addition to sample collection, transport and inactivation, nucleic acid detection products are mainly divided into two steps in the detection process, namely Nucleic Acid Extraction Reagent and amplification detection. The amplification detection part mainly depends on the purity of Safe Nucleic Acid Extraction Reagent in the previous step, as well as the performance of the amplification instrument, so the difference of nucleic acid detection products itself is the key to the "nucleic acid extraction" step. Nucleic Acid Extraction Reagent technology can also be divided into 2 parts, < sample cleavage > and < nucleic acid purification >, the common sample cleavage technology on the market at present has < physical heating cleavage >, < protease K cleavage >, < one-step nucleic acid extraction >, nucleic acid purification technology has < gradient centrifuge method >, < silica gel column method >, < magnetic bead method >. Therefore, different nucleic acid extraction techniques, sample cracking and nucleic acid purification procedures are different, and the operation complexity is different, as well as the extraction efficiency and purity of nucleic acid. However, complex operation is more likely to lead to aerosol contamination, bringing the risk of cross infection. Therefore, the selection of appropriate extraction technology is very important for the current epidemic prevention and control.
Simple, rapid and Safe Nucleic Acid Extraction Reagent scheme:
One-step RNA technology "One-step RNA technology" can not only provide a complete nucleic acid extraction + amplification scheme, but also improve the detection efficiency. The extraction technology is divided into two steps: sample lysis and Reliable Nucleic Acid Extraction Reagent purification: ① The first step is sample lysis. The common sample pyrolysis techniques < physical heating pyrolysis > and < protease K pyrolysis > all require heating, leading to a series of problems such as aerosol contamination. "One-step RNA technology" uses the "one-step nucleic acid extraction" technology, without heating and boiling, simple, fast, and economical. ② The second step, Nucleic Acid Extraction Reagent purification. In recent years, in vitro diagnostic industry technology advances by leaps and bounds, from < gradient centrifugation method >, < silica gel column method >, to < magnetic bead method > progress, step by step to reduce the loss of nucleic acid, simplify the steps, and improve the purity and efficiency of nucleic acid purification. Due to the technical advantages of the first step of sample cracking, one-step RNA technology can completely eliminate this step and achieve "no purification". The sensitivity of the novel coronavirus nucleic acid detection reagent using this extraction technology can reach 200copies/ml.
For inspection staff, the magnetic bead method may be more popular. "Normal temperature magnetic bead technology" is also under the premise of no heating, compared with other Nucleic Acid Extraction Reagent technology more simplified operation steps, only one washing, can reduce the risk of aerosol pollution, reduce the chance of cross infection; No ethanol was used and there was no risk of ethanol residue inhibiting the downstream PCR reaction. Moreover, it has higher purity, sensitivity and accuracy, which can make the novel coronavirus nucleic acid detection reagent equipped with this Nucleic Acid Extraction Reagent technology more sensitive. It can be used to accurately guide antiviral therapy, evaluate the efficacy of antiviral therapy, judge the treatment endpoint, and provide reliable auxiliary diagnosis basis for clinical.
